Bi 530 Theory of Recombinant DNA Techniques

Lectures on the principles and theory of recombinant DNA and molecular cloning techniques. Topics will cover use of restriction and other DNA modifying enzymes, host-vector systems, DNA fragment and plasmid isolation techniques, gene mapping, subcloning techniques, in vitro mutagenesis, cDNA and genomic cloning, screening of clones, blot hybridizations, DNA transfection and use of reporter genes, DNA sequencing and PCR.